NPTN Polyclonal antibody

NPTN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28022-1-AP

Synonyms

DKFZp686L2477, GP55, GP65, neuroplastin, NPTN, SDFR1, SDR 1, SDR1

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Validation Data Gallery

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  WB analysis of C6 using 28022-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 28022-1-AP (NPTN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28022-1-AP targets NPTN in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPTN fusion protein Ag27752
• Full Name: neuroplastin
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC030773
• Gene Symbol: NPTN
• Gene ID (NCBI): 27020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460)