NOTUM Monoclonal antibody

NOTUM Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6D5

Cat no : 66956-1-Ig

Synonyms

NOTUM, Protein notum homolog



Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, T-47D cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66956-1-Ig targets NOTUM in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOTUM fusion protein Ag6338
Full Name notum pectinacetylesterase homolog (Drosophila)
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC060882
Gene Symbol NOTUM
Gene ID (NCBI) 147111
RRIDAB_2882279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOTUM antibody 66956-1-IgDownload protocol
IHC protocol for NOTUM antibody 66956-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols