CoraLite® Plus 488-conjugated NNT Monoclonal antibody

NNT Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5H6

Cat no : CL488-68194

Synonyms

NNT

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68194

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NNT antibody (CL488-68194, Clone: 1F5H6 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68194 targets NNT in IF applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NNT fusion protein Ag4259
• Full Name: nicotinamide nucleotide transhydrogenase
• Calculated Molecular Weight: 1085 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC032370
• Gene Symbol: NNT
• Gene ID (NCBI): 23530
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).