NGRN Monoclonal antibody

NGRN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C2C9

Cat no : 68593-1-Ig

Synonyms

DSC92, FI58G, Neugrin, NGRN

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Validation Data Gallery

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  WB analysis using 68593-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68593-1-Ig (NGRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68593-1-Ig targets NGRN in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NGRN fusion protein Ag6790
• Full Name: neugrin, neurite outgrowth associated
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC001682
• Gene Symbol: NGRN
• Gene ID (NCBI): 51335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.