NGRN Polyclonal antibody

• Featured Product
• KD/KO Validated

NGRN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14885-1-AP

Synonyms

DSC92, FI58G, Neugrin, NGRN

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Validation Data Gallery

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  WB analysis of mouse liver using 14885-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14885-1-AP (NGRN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14885-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14885-1-AP (NGRN Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human brain tissue, human heart tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

14885-1-AP targets NGRN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGRN fusion protein Ag6661
• Full Name: neugrin, neurite outgrowth associated
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC001682
• Gene Symbol: NGRN
• Gene ID (NCBI): 51335
• RRID: AB_2878090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.

Publications

Species	Application	Title
human	WB,IF	Cell Metab; A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.; Authors - Jason D Arroyo ; KO Validated
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	EMBO Rep; A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.; Authors - Hana Antonicka
		J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart