NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30229-1-AP

Synonyms

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

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Validation Data Gallery

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  WB analysis using 30229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30229-1-AP targets NGEF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGEF fusion protein Ag30842
• Full Name: neuronal guanine nucleotide exchange factor
• Calculated Molecular Weight: 710 aa, 82 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC031573
• Gene Symbol: NGEF
• Gene ID (NCBI): 25791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGEF enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis.