NGB Polyclonal antibody

NGB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13499-1-AP

Synonyms

neuroglobin, NGB



Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13499-1-AP targets NGB in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGB fusion protein Ag4325
Full Name neuroglobin
Calculated Molecular Weight 151 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC032509
Gene Symbol NGB
Gene ID (NCBI) 58157
RRIDAB_2115391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NGB antibody 13499-1-APDownload protocol
IHC protocol for NGB antibody 13499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Diabetes

Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq.

Authors - Yinqiu Wang
WB,IHC,IF

Cell Death Dis

Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases.

Authors - Haixia Wen
IHC

Biomolecules

Intravitreal Neuroglobin Mitigates Primate Experimental Glaucomatous Structural Damage in Association with Reduced Optic Nerve Microglial and Complement 3-Astrocyte Activation

Authors - Anita S Y Chan
mouseWB

Antioxidants (Basel)

Prunus cerasoides Extract and Its Component Compounds Upregulate Neuronal Neuroglobin Levels, Mediate Antioxidant Effects, and Ameliorate Functional Losses in the Mouse Model of Cerebral Ischemia.

Authors - So-Dam Kim
humanFC

Cells

Connecting the Dots in the Neuroglobin-Protein Interaction Network of an Unstressed and Ferroptotic Cell Death Neuroblastoma Model.

Authors - Zoë P Van Acker
WB

Front Neurosci

PMID: 31191229

Authors - Fan Yang