NF45 Polyclonal antibody

• Featured Product
• KD/KO Validated

NF45 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14714-1-AP

Synonyms

ILF2, NF45, PRO3063

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Validation Data Gallery

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  WB analysis using 14714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14714-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14714-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14714-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 14714-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14714-1-AP (NF45 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 14714-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14714-1-AP (NF45 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, NIH/3T3 cells, Jurkat cells, rat brain tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14714-1-AP targets NF45 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF45 fusion protein Ag6436
• Full Name: interleukin enhancer binding factor 2, 45kDa
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC000382
• Gene Symbol: ILF2
• Gene ID (NCBI): 3608
• RRID: AB_2280445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

Publications

Species	Application	Title
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
		Analyst; An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.; Authors - Yu Yanyan Y
human	WB,IF	Virol J; Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.; Authors - Xuexia Wen
human	WB	J Exp Clin Cancer Res; LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex; Authors - Dandan Li
human	WB	Nat Cell Biol; Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing; Authors - Guokai Yan