NEU4 Polyclonal antibody

NEU4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12995-1-AP

Synonyms

N acetyl alpha neuraminidase 4, NEU4, sialidase 4

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Validation Data Gallery

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  WB analysis using 12995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12995-1-AP (NEU4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 12995-1-AP

  IP result of anti-NEU4 (IP:12995-1-AP, 4ug; Detection:12995-1-AP 1:500) with L02 cells lysate 1400ug.

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  IHC staining of human placenta using 12995-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12995-1-AP (NEU4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12995-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12995-1-AP (NEU4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

12995-1-AP targets NEU4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEU4 fusion protein Ag3657
• Full Name: sialidase 4
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC012899
• Gene Symbol: NEU4
• Gene ID (NCBI): 129807
• RRID: AB_2149206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEU4, also named as LP5125, belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. It may function in lysosomal catabolism of sialylated glycoconjugates. NEU4 has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids.

Publications

Species	Application	Title
human	WB	J Biol Chem; Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles.; Authors - Bilyy Rostyslav O RO
mouse	WB,IP	Cell Signal; A novel epidermal growth factor receptor-signaling platform and its targeted translation in pancreatic cancer.; Authors - Gilmour Alanna M AM
rat	WB,IP	Cell Signal; PMID: 24583283; Authors - Farah Alghamdi
rat	WB,IF	Cell Signal; Neu1 sialidase and matrix metalloproteinase-9 cross-talk is essential for neurotrophin activation of Trk receptors and cellular signaling.; Authors - Jayanth Preethi P
mouse	WB,IF	Glycoconj J; Dependence of pathogen molecule-induced toll-like receptor activation and cell function on Neu1 sialidase.; Authors - Amith Schammim Ray SR
human	WB	Glycoconj J; Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9.; Authors - Finlay Trisha M TM