NECAP1 Polyclonal antibody

NECAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17906-1-AP

Synonyms

NECAP 1, NECAP endocytosis associated 1, NECAP1

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Validation Data Gallery

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  WB analysis using 17906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17906-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17906-1-AP (NECAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17906-1-AP

  IP result of anti-NECAP1 (IP:17906-1-AP, 4ug; Detection:17906-1-AP 1:300) with mouse brain tissue lysate 6000 ug.

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  IHC staining of human kidney using 17906-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17906-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17906-1-AP (NECAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17906-1-AP targets NECAP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NECAP1 fusion protein Ag12283
• Full Name: NECAP endocytosis associated 1
• Calculated Molecular Weight: 275 aa, 30 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC110876
• Gene Symbol: NECAP1
• Gene ID (NCBI): 25977
• RRID: AB_2149177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	J Med Genet; NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.; Authors - Anas M Alazami
mouse		Sci Rep; Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.; Authors - Ermes Candiello