NECAB1 Polyclonal antibody

NECAB1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16477-1-AP

Synonyms

EFCBP1, NECAB1, STIP 1

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Validation Data Gallery

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  IHC staining of mouse brain using 16477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16477-1-AP (NECAB1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16477-1-AP (NECAB1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 16477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NECAB1 antibody (16477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16477-1-AP targets NECAB1 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NECAB1 fusion protein Ag9591
• Full Name: N-terminal EF-hand calcium binding protein 1
• Calculated Molecular Weight: 351 aa, 40 kDa
• GenBank Accession Number: BC016340
• Gene Symbol: NECAB1
• Gene ID (NCBI): 64168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NECAB1, also named as EFCBP1, is a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains Short Communication.