NDUFAF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFAF4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26003-1-AP

Synonyms

C6orf66, HRPAP20, HSPC125, My013, NDUFAF4

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Validation Data Gallery

• 

  WB analysis using 26003-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26003-1-AP (NDUFAF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26003-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26003-1-AP (NDUFAF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 26003-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26003-1-AP (NDUFAF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 26003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26003-1-AP (NDUFAF4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26003-1-AP (NDUFAF4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 26003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26003-1-AP (NDUFAF4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 26003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26003-1-AP (NDUFAF4 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, K-562 cells
• Positive IHC detected in: human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

The immunogen of 26003-1-AP is NDUFAF4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFAF4 fusion protein Ag23365
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC039464
• Gene Symbol: NDUFAF4
• Gene ID (NCBI): 29078
• RRID: AB_2880329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFAF4 is a protein that in humans is encoded by the C6ORF66 gene. NDUFAF4 is an assembly factor of complex I. Mutation of this gene has been associated with congenital disorders of the mitochondrial respiratory chain (PMID: 18179882). NDUFAF4 was previously shown to promote breast cancer cell invasiveness. NDUFAF4 is a 20.2 kDa mitochondrial protein. Catalog#26003-1-AP specifically recognises the 20 kDa protein.

Publications

Species	Application	Title
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang
mouse	WB	Stem Cell Res Ther; Mitochondrial activity regulates the differentiation of skin-derived mesenchymal stem cells into brown adipocytes to contribute to hypertension.; Authors - Wenda Xi
human	WB	Virus Genes; The host cellular protein Ndufaf4 interacts with the vesicular stomatitis virus M protein and affects viral propagation.; Authors - Wei Pan ; KD Validated