NDUFA7 Polyclonal antibody

NDUFA7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15300-1-AP

Synonyms

B14.5a, CI B14.5a, Complex I B14.5a, NDUFA7

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Validation Data Gallery

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  WB analysis of mouse heart using 15300-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15300-1-AP (NDUFA7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15300-1-AP targets NDUFA7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFA7 fusion protein Ag7558
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC003102
• Gene Symbol: NDUFA7
• Gene ID (NCBI): 4701
• RRID: AB_2878123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFA7(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7) is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	WB	Nat Commun; Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS.; Authors - Xiaojian Shi
human	WB	Brain Pathol; Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia.; Authors - Paula Garcia-Esparcia