NDUFA6 Polyclonal antibody

NDUFA6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15445-1-AP

Synonyms

B14, CI B14, Complex I B14, LYR motif containing protein 6, LYRM6, NADHB14, NDUFA6

---

Validation Data Gallery

• 

  WB analysis of human heart using 15445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15445-1-AP (NDUFA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15445-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15445-1-AP (NDUFA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, rat heart tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15445-1-AP targets NDUFA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFA6 fusion protein Ag7593
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC002772
• Gene Symbol: NDUFA6
• Gene ID (NCBI): 4700
• RRID: AB_2267065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFA6 (NADH:Ubiquinone Oxidoreductase Subunit A6), also called LYR motif-containing protein 6 (LYRM6), or Complex I-B14 (CI-B14), or NADH-ubiquinone oxidoreductase B14 subunit, is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). NDUFA6 participates in the regulation of adipogenic differentiation.