DAP13/NDUFA12 Polyclonal antibody

• Featured Product
• KD/KO Validated

DAP13/NDUFA12 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15793-1-AP

Synonyms

B17.2, CI B17.2, CIB17.2, Complex I B17.2, DAP13, NDUFA12

---

Validation Data Gallery

• 

  WB analysis using 15793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15793-1-AP (DAP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15793-1-AP (DAP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 15793-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15793-1-AP (DAP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15793-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15793-1-AP (DAP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15793-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15793-1-AP (DAP13 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse kidney tissue, mouse heart tissue, rat kidney tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15793-1-AP targets DAP13/NDUFA12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAP13/NDUFA12 fusion protein Ag8501
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
• Calculated Molecular Weight: 17 kDa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC005936
• Gene Symbol: NDUFA12
• Gene ID (NCBI): 55967
• RRID: AB_2150475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
human	WB	Hum Mutat; Novel NDUFA12 variants are associated with isolated complex I defect and variable clinical manifestation.; Authors - Alessandra Torraco