Kiz Polyclonal antibody

Kiz Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 21177-1-AP

Synonyms

C20orf19, Centrosomal protein kizuna, HT013, KIZ, NCRNA00153, non protein coding RNA 153, PLK1S1, Polo like kinase 1 substrate 1

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Validation Data Gallery

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  WB analysis of HeLa using 21177-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21177-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21177-1-AP

  IP result of anti-Kiz (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human testis using 21177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 21177-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

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  IF Staining of hTERT-RPE1 using 21177-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Kiz antibody (21177-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

The immunogen of 21177-1-AP is Kiz Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kiz fusion protein Ag15454
• Full Name: non-protein coding RNA 153
• Calculated Molecular Weight: 673 aa, 75 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC045826
• Gene Symbol: NCRNA00153
• Gene ID (NCBI): 55857
• RRID: AB_10755292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (05-15-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), Kizuna (in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 5' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)

Kiz Antibody Immunofluorescence validation (1:200 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:21177-1-AP)