p47 phox Polyclonal antibody

p47 phox Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28187-1-AP

Synonyms

FLJ79451, NCF 1, NCF 47K, NCF1, NCF1A, Neutrophil cytosol factor 1, neutrophil cytosolic factor 1, Nox organizer 2, Nox organizing protein 2, NOXO2, p47 phox, p47phox, p47-phox, SH3PXD1A

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Validation Data Gallery

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  WB analysis using 28187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28187-1-AP (p47 phox antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 28187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28187-1-AP (p47 phox antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28187-1-AP (p47 phox antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-937 cells, Raji cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28187-1-AP targets p47 phox in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p47 phox fusion protein Ag28089
• Full Name: neutrophil cytosolic factor 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC002816
• Gene Symbol: NCF1
• Gene ID (NCBI): 653361
• RRID: AB_2881084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang
rat	WB	Drug Des Devel Ther; Artemisinin Improves Acetylcholine-Induced Vasodilatation in Rats with Primary Hypertension; Authors - Xuanxuan Liu