CoraLite® Plus 647-conjugated NCAPH Monoclonal antibody

NCAPH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3D2F11

Cat no : CL647-67655

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

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Validation Data Gallery

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  IF Staining of human liver cancer using CL647-67655

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 647 NCAPH antibody (CL647-67655, Clone: 3D2F11 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67655 targets NCAPH in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NCAPH fusion protein Ag27748
• Full Name: non-SMC condensin I complex, subunit H
• Calculated Molecular Weight: 741 aa, 83 kDa
• GenBank Accession Number: BC024211
• Gene Symbol: NCAPH
• Gene ID (NCBI): 23397
• RRID: AB_2920297
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )