NBAS Polyclonal antibody

NBAS Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 14683-1-AP

Synonyms

NAG, NBAS

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Validation Data Gallery

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  IHC staining of mouse brain using 14683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14683-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14683-1-AP (NBAS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 14683-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14683-1-AP (NBAS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 14683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 14683-1-AP (NBAS antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14683-1-AP targets NBAS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBAS fusion protein Ag6407
• Full Name: neuroblastoma amplified sequence
• Calculated Molecular Weight: 269 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC051792
• Gene Symbol: NBAS
• Gene ID (NCBI): 51594
• RRID: AB_1959590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBAS, also known as neuroblastoma amplified (NAG) gene, was identified as a gene that is co-amplified with the N-myc (MYCN) gene in neuroblastoma cell lines. NBAS is a factor of the nonsense-mediated mRNA decay (NMD), which is a highly conserved surveillance pathway that allows the organism to respond to cellular and environmental stress.(PMID: 23828042)

Publications

Species	Application	Title
human	WB	Bone; Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1.; Authors - Eleonora Palagano
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-27-2021)

This antibody primarily recognised two bands around 150 and 100 kd. The calculated molecular weight is over 250 kd.

• Applications: Western Blot
• Cell Tissue Type: MEF