NANOS3 Polyclonal antibody

NANOS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21679-1-AP

Synonyms

Nanos homolog 3, nanos homolog 3 (Drosophila), NANOS1L, NANOS3, NOS 3, NOS3



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21679-1-AP is NANOS3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANOS3 fusion protein Ag16014
Full Name nanos homolog 3 (Drosophila)
Calculated Molecular Weight 192 aa, 21 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC101209
Gene Symbol NANOS3
Gene ID (NCBI) 342977
RRIDAB_10859250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.

Protocols

Product Specific Protocols
WB protocol for NANOS3 antibody 21679-1-APDownload protocol
IHC protocol for NANOS3 antibody 21679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acids Res

Regulation of both transcription and RNA turnover contribute to germline specification.

Authors - Kun Tan
mouse,ratWB,IF

Environ Pollut

Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling

Authors - Yifan Hong
humanWB,IHC,IF

J Pathol

The human NANOS3 gene contributes to lung tumour invasion by inducing epithelial-mesenchymal transition.

Authors - Simon Grelet
ratWB

Exp Cell Res

Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis.

Authors - Yuanping Cao
humanWB,IHC

BMC Cancer

A new mouse model to study the role of ectopic Nanos3 expression in cancer.

Authors - Vanessa Andries
mouseWB

J Ethnopharmacol

Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC (soluble guanylate cyclase) - cGMP (cyclic guanosine monophosphate) - PKG (protein kinase G) pathway

Authors - Liqian Chen