NAGS Polyclonal antibody

NAGS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 21566-1-AP

Synonyms

AGAS, Amino acid acetyltransferase, ARGA, N acetylglutamate synthase, NAGS

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Validation Data Gallery

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  WB analysis of HepG2 using 21566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21566-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21566-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21566-1-AP (NAGS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 21566-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21566-1-AP (NAGS antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21566-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 21566-1-AP

  Immunofluorescent analysis of Hela cells, using NAGS antibody 21566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, L02 cells
• Positive IHC detected in: human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21566-1-AP targets NAGS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAGS fusion protein Ag16073
• Full Name: N-acetylglutamate synthase
• Calculated Molecular Weight: 534 aa, 58 kDa
• Observed Molecular Weight: 58-66 kDa
• GenBank Accession Number: BC111713
• Gene Symbol: NAGS
• Gene ID (NCBI): 162417
• RRID: AB_10733886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).

Publications

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Heaji (Verified Customer) (09-30-2019)

As shown in the image the antibody recognizes a strong band of the wrong size in the NAGS knockout model -- I don't think this works as intended. Thank you.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver and intestine

NAGS Antibody Western Blot, validation (1:2000 dilution) in mouse liver and intestine (Cat no:21566-1-AP)