MYST2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYST2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13751-1-AP

Synonyms

HBO1, HBOA, KAT7, MYST 2, MYST2

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Validation Data Gallery

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  WB analysis of HeLa using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13751-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13751-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13751-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13751-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13751-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13751-1-AP

  IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 13751-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

13751-1-AP targets MYST2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYST2 fusion protein Ag4693
• Full Name: MYST histone acetyltransferase 2
• Calculated Molecular Weight: 611 aa, 71 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC032640
• Gene Symbol: MYST2
• Gene ID (NCBI): 11143
• RRID: AB_2266703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

Publications

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li ; KO Validated
human	WB,IF,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Cancer Res; Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.; Authors - Mylinh T Duong
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
human	WB	Mol Cell Proteomics; Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.; Authors - Ding Wang
human	WB	Mol Cell Biol; HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells.; Authors - Andrew J Kueh