MYO19 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYO19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23906-1-AP

Synonyms

MYO19, MYOHD1, myosin XIX

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Validation Data Gallery

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  WB analysis of A549 using 23906-1-AP

  WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

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  WB analysis of HeLa using 23906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 23906-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 23906-1-AP

  Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, COS-7 cells
• Positive IHC detected in: human kidney tissue, rat kidney tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23906-1-AP targets MYO19 in WB, IHC, IF, ELISA applications and shows reactivity with human, monkey, rat samples.

• Tested Reactivity: human, monkey, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO19 fusion protein Ag20995
• Full Name: myosin XIX
• Calculated Molecular Weight: 970 aa, 109 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: BC008900
• Gene Symbol: MYO19
• Gene ID (NCBI): 80179
• RRID: AB_2879357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.