MYO16 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYO16 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25104-1-AP

Synonyms

KIAA0865, MYO16, Myo16b, myosin XVI, MYR8, Unconventional myosin 16

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Validation Data Gallery

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  WB analysis of rat brain using 25104-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25104-1-AP (MYO16 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

The immunogen of 25104-1-AP is MYO16 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO16 fusion protein Ag18798
• Full Name: myosin XVI
• Calculated Molecular Weight: 1858 aa, 206 kDa
• Observed Molecular Weight: 200-240 kDa
• GenBank Accession Number: BC146791
• Gene Symbol: MYO16
• Gene ID (NCBI): 23026
• RRID: AB_2879898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.

Publications

Species	Application	Title
mouse	WB	Front Cell Neurosci; Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization.; Authors - Mona Katrin Roesler ; KO Validated
human	WB	Mol Biol Cell; The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis.; Authors - Shalini Menon ; KD Validated