MYO16 Polyclonal antibody

MYO16 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18828-1-AP

Synonyms

KIAA0865, MYO16, Myo16b, myosin XVI, MYR8, Unconventional myosin 16

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Validation Data Gallery

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  IHC staining of human bladder using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 18828-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 18828-1-AP (MYO16 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 18828-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 18828-1-AP (MYO16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human bladder tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18828-1-AP targets MYO16 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin XVI
• Calculated Molecular Weight: 1858 aa, 206 kDa
• Observed Molecular Weight: 123 kDa, 210 kDa, 106 kDa
• GenBank Accession Number: BC146791
• Gene Symbol: MYO16
• Gene ID (NCBI): 23026
• RRID: AB_10858634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO16, also named as KIAA0865, MYO16B, is actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. MYO16 may be involved in targeting of the catalytic subunit of protein phosphatase 1 during brain development. The antibody can recognize isoform1, isoform2 and isoform3 of MYO16.