MYL6 Monoclonal antibody

• Featured Product
• KD/KO Validated

MYL6 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G4C9

Cat no : 68142-1-Ig

Synonyms

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

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Validation Data Gallery

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  WB analysis using 68142-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68142-1-Ig

  WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells.

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  IHC staining of human colon using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 68142-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68142-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig heart tissue, A549 cells, rabbit heart tissue, rat heart tissue, mouse heart tissue
• Positive IHC detected in: human colon tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human kidney tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68142-1-Ig targets MYL6 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYL6 fusion protein Ag13091
• Full Name: myosin, light chain 6, alkali, smooth muscle and non-muscle
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17-25 kDa
• GenBank Accession Number: BC006781
• Gene Symbol: MYL6
• Gene ID (NCBI): 4637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).