MYL4 Monoclonal antibody

MYL4 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

2F5C1

Cat no : 67533-1-Ig

Synonyms

ALC1, AMLC, GT1, MLC1, MYL4, Myosin light chain 4, PRO1957

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Validation Data Gallery

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  WB analysis using 67533-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 67533-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 67533-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of C2C12 using 67533-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: pig heart tissue, rat heart tissues, mouse heart tissues
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue
• Positive FC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

67533-1-Ig targets MYL4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYL4 fusion protein Ag19244
• Full Name: myosin, light chain 4, alkali; atrial, embryonic
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC030228
• Gene Symbol: MYL4
• Gene ID (NCBI): 4635
• RRID: AB_2882752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.

Publications

Species	Application	Title
human	IF	Front Cell Dev Biol; HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.; Authors - Emmi Helle
rat	WB	J Proteome Res; Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts; Authors - Bridgette Hartley
mouse	WB,IF	Genes (Basel); Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs; Authors - Xueli Xu ; KD Validated
pig	WB	Gene; Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size; Authors - Shixiong Dong
mouse	WB	Cell Biosci; Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers; Authors - Lei Bai
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C