MYB/c-Myb Monoclonal antibody

MYB/c-Myb Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4A5

Cat no : 68542-1-Ig

Synonyms

c myb, c myb_CDS, Cmyb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb

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Validation Data Gallery

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  WB analysis using 68542-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68542-1-Ig (MYB/c-Myb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 68542-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MYB/c-Myb (68542-1-Ig, Clone:2C4A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68542-1-Ig targets MYB/c-Myb in WB, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Full Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 640 aa, 72 kDa
• Observed Molecular Weight: 40-50 kDa, 72-80 kDa
• GenBank Accession Number: BC064955
• Gene Symbol: MYB
• Gene ID (NCBI): 4602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.