MXD1 Polyclonal antibody

MXD1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19547-1-AP

Synonyms

MAD, MAD1, MAX dimerization protein 1, Max dimerizer 1, MXD1, Protein MAD

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Validation Data Gallery

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  WB analysis of HepG2 using 19547-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19547-1-AP (MXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 19547-1-AP

  IP result of anti-MXD1 (IP:19547-1-AP, 4ug; Detection:19547-1-AP 1:500) with HepG2 cells lysate 3000ug.

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ChIP: See 1 publications below

Product Information

19547-1-AP targets MXD1 in WB, IP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: MAX dimerization protein 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_002357
• Gene Symbol: MXD1
• Gene ID (NCBI): 4084
• RRID: AB_10641995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXD1, also named as MAD, is a transcriptional repressor. MAD1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX. There're two isoforms of MXD1 protein.

Publications

Species	Application	Title
human	chIP	J Biol Chem; Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1.; Authors - Jianyin Long