MUC20 Polyclonal antibody

MUC20 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13380-1-AP

Synonyms

KIAA1359, MUC 20, MUC20, Mucin 20

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Validation Data Gallery

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  WB analysis of human liver using 13380-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13380-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13380-1-AP (MUC20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13380-1-AP

  IP result of anti-MUC20 (IP:13380-1-AP, 4ug; Detection:13380-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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Tested Applications

• Positive WB detected in: human liver tissue, human kidney tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13380-1-AP targets MUC20 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUC20 fusion protein Ag4205
• Full Name: mucin 20, cell surface associated
• Calculated Molecular Weight: 55 kDa, 71 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC029267
• Gene Symbol: MUC20
• Gene ID (NCBI): 200958
• RRID: AB_2235526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis; Authors - Xiaobin Wang