MTSS1 Polyclonal antibody

MTSS1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13995-1-AP

Synonyms

KIAA0429, metastasis suppressor 1, Metastasis suppressor YGL 1, MIM, MIMA, MIMB, Missing in metastasis protein, MTSS1

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Validation Data Gallery

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  WB analysis using 13995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13995-1-AP (MTSS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13995-1-AP (MTSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13995-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1 antibody (13995-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

13995-1-AP targets MTSS1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTSS1 fusion protein Ag5080
• Full Name: metastasis suppressor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC023998
• Gene Symbol: MTSS1
• Gene ID (NCBI): 9788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTSS1 (metastasis suppressor 1), also known as MIM (missing-in-metastasis), MIM-B, BEG4 (Basal cell carcinoma-enriched gene 4) or KIAA0429, is one of the defining members of the I-BAR family of negative membrane curvature-sensing proteins first identified as being deleted in metastatic bladder cancer. MTSS1 over-expression is associated with enhanced cell migration, resulting in tumorigenesis, invasion and metastasis (PMID: 27230279). It is reported that the expression of MTSS1 is associated with poor prognosis in various cancers,such as bladder, gastric, breast and so on (PMID: 32052679).

Publications

Species	Application	Title
human	WB,IF	Cytoskeleton (Hoboken); Metastasis suppressor 1 interacts with α-actinin 4 to affect its localization and regulate formation of membrane ruffling.; Authors - Lijun Liang ; KD Validated