MTA2 Monoclonal antibody

• Featured Product
• KD/KO Validated

MTA2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

2G12G4

Cat no : 66195-1-Ig

Synonyms

Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID

---

Validation Data Gallery

• 

  WB analysis using 66195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66195-1-Ig (MTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66195-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTA2 antibody (66195-1-Ig, Clone: 2G12G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66195-1-Ig

  1X10^6 HepG2 cells were stained with .2ug MTA2 antibody (66195-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

---

Tested Applications

• Positive WB detected in: SW480 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: mouse brain tissue, human lung tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:125-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

66195-1-Ig targets MTA2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MTA2 fusion protein Ag11713
• Full Name: metastasis associated 1 family, member 2
• Calculated Molecular Weight: 668 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC053650
• Gene Symbol: MTA2
• Gene ID (NCBI): 9219
• RRID: AB_2877118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publications

Species	Application	Title
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB,IF	Theranostics; LncRNA-MTA2TR functions as a promoter in pancreatic cancer via driving deacetylation-dependent accumulation of HIF-1α.; Authors - Zhu Zeng ; KD Validated
human	WB	Oncol Lett; Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.; Authors - Shanshan Li
human	WB	Cancers (Basel); ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma; Authors - Yayuan Zheng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao