MT1X Polyclonal antibody

MT1X Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17172-1-AP

Synonyms

metallothionein 1X, Metallothionein IX, MT 1l, MT 1X, MT IX, MT1, MT1X



Tested Applications

Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17172-1-AP targets MT1X in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MT1X fusion protein Ag10711
Full Name metallothionein 1X
Calculated Molecular Weight 46 aa, 5 kDa
GenBank Accession NumberBC032131
Gene Symbol MT1X
Gene ID (NCBI) 4501
RRIDAB_2878354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MT1X antibody 17172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Autophagy

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Authors - Wenjing Xiao
humanIHC

Cancer Res

Intratumoral Copper Modulates PD-L1 Expression and Influences Tumor Immune Evasion.

Authors - Florida Voli
humanIHC

J Cell Mol Med

Comprehensive analysis of partial epithelial mesenchymal transition-related genes in hepatocellular carcinoma.

Authors - Yu Lei
humanIHC

Cancer Biol Ther

MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.

Authors - Xiangke Xin
humanIHC

Cell Death Dis

Comparison of the major cell populations among osteoarthritis, Kashin-Beck disease and healthy chondrocytes by single-cell RNA-seq analysis.

Authors - Xi Wang
chickenIHC

Poult Sci

The capability of L-carnitine-mediated antioxidant on cock during aging: evidence for the improved semen quality and enhanced testicular expressions of GnRH1, GnRHR, and melatonin receptors MT 1/2.

Authors - Abdelmotaleb A Elokil

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Adrianna (Verified Customer) (10-31-2022)

There is a lot of unspecific bands with this antibody and I cannot see the actual band being brighter. I have used it at 1:1000 dilution, and incubated ON at 4 deg C. Perhaps I should try a different dilution / lysate concentration.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7