MSC Polyclonal antibody

MSC Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25028-1-AP

Synonyms

ABF 1, ABF1, Activated B cell factor 1, bHLHa22, MSC, Musculin, MYOR

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Validation Data Gallery

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  WB analysis of mouse spleen using 25028-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 25028-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 25028-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25028-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25028-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25028-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25028-1-AP is MSC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSC fusion protein Ag13489
• Full Name: musculin (activated B-cell factor-1)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006313
• Gene Symbol: MSC
• Gene ID (NCBI): 9242
• RRID: AB_2879857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSC, also named as Musculin, is a 206 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. MSC localizes in the nucleus and as a transcription repressor is capable of inhibiting the transactivation capability of TCF3/E47. MSC may play a role in regulating antigen-dependent B-cell differentiation.