MRPL55 Polyclonal antibody

MRPL55 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17679-1-AP

Synonyms

AAVG5835, L55mt, L55nt, MRP L55, MRPL55, PRO19675

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Validation Data Gallery

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  IP experiment of HEK-293 using 17679-1-AP

  IP result of anti-MRPL55 (IP:17679-1-AP, 4ug; Detection:17679-1-AP 1:600) with HEK-293 cells lysate 1680 ug.

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  WB analysis of HEK-293 using 17679-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17679-1-AP (MRPL55 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17679-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17679-1-AP (MRPL55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17679-1-AP is MRPL55 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL55 fusion protein Ag11716
• Full Name: mitochondrial ribosomal protein L55
• Calculated Molecular Weight: 128 aa, 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC052806
• Gene Symbol: MRPL55
• Gene ID (NCBI): 128308
• RRID: AB_2878427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL55, also named as L55mt and MRP-L55, is a component of the mitochondrial ribosome large subunit (39S). It acts dynamically in the cell during development. The MW of MRPL55 is about 13 kDa.

Publications

Species	Application	Title
human	WB	Mol Biol Cell; Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.; Authors - Katarzyna Justyna Chojnacka