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MRPL34 Polyclonal antibody

MRPL34 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15233-1-AP

Synonyms

L34mt, MRP L34, MRPL34

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPL34 Polyclonal antibody (15233-1-AP)

    IHC staining of human liver cancer using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15233-1-AP (MRPL34 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPL34 Polyclonal antibody (15233-1-AP)

    IHC staining of human liver cancer using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15233-1-AP (MRPL34 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL34 Polyclonal antibody (15233-1-AP)

    IF Staining of HepG2 using 15233-1-AP

    Immunofluorescent analysis of HepG2 cells using 15233-1-AP (MRPL34 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15233-1-AP targets MRPL34 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL34 fusion protein Ag7173
Full Name mitochondrial ribosomal protein L34
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC000071
Gene Symbol MRPL34
Gene ID (NCBI) 64981
RRIDAB_2145915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MRPL34 antibody 15233-1-APDownload protocol
IF protocol for MRPL34 antibody 15233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols