MRPL13 Polyclonal antibody

MRPL13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16241-1-AP

Synonyms

L13, L13A, L13mt, MRP L13, MRPL13, RPL13, RPML13

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Validation Data Gallery

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  WB analysis using 16241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16241-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16241-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16241-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 16241-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16241-1-AP

  IP result of anti-MRPL13 (IP:16241-1-AP, 4ug; Detection:16241-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, mouse lung tissue, Jurkat cells, COS-7 cells, rat lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

16241-1-AP targets MRPL13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL13 fusion protein Ag9105
• Full Name: mitochondrial ribosomal protein L13
• Calculated Molecular Weight: 178 aa, 21 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC009190
• Gene Symbol: MRPL13
• Gene ID (NCBI): 28998
• RRID: AB_2145705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRPL13, also named as 39S ribosomal protein L13, mitochondrial or L13mt, is a 178 amino acid protein, which belongs to the ribosomal protein L13P family. MRPL13 localizes in the mitochondrion and interacts with OXA1L. MRPL13 is involved in mitochondrial proteins biosynthesis, ribosomal structure and biogenesis.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.; Authors - Surovtseva Yulia V YV
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	J Cell Sci; CLUH couples mitochondrial distribution to the energetic and metabolic status.; Authors - Jamal Wakim
human	WB	Life (Basel); Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease; Authors - Paola Loguercio Polosa