MPZL3 Polyclonal antibody

MPZL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25513-1-AP

Synonyms

MPZL3, myelin protein zero like 3

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 25513-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25513-1-AP (MPZL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25513-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25513-1-AP (MPZL3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

---

Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25513-1-AP is MPZL3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPZL3 fusion protein Ag21905
• Full Name: myelin protein zero-like 3
• Calculated Molecular Weight: 235 aa, 26 kDa
• Observed Molecular Weight: 70 kDa, 28 kDa
• GenBank Accession Number: BC113586
• Gene Symbol: MPZL3
• Gene ID (NCBI): 196264
• RRID: AB_2880111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPZL3 (Myelin protein zero-like protein 3) is a member of the myelin P0 protein family. The human MPZL3 gene is located at chromosome 11q23.3, and encodes a 235-amino acid polypeptide with an Immunoglobulin V-type (IgV) domain. MPZL3 protein is a membrane adhesion protein. It may be involved in immune function (PMID: 19054061). This polyclonal antibody raised against 32-235aa of human MPZL3 reveals bands of 28 kDa and 70 kDa, which could be endogenous MPZL3 of unmodified form and post-translational modified form, respectively (PMID: 17273165).

Publications

Species	Application	Title
human	WB	Cell Mol Life Sci; Met-HER3 crosstalk supports proliferation via MPZL3 in MET-amplified cancer cells.; Authors - Yaakov E Stern