MON1B Monoclonal antibody

• Featured Product
• KD/KO Validated

MON1B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E7C6

Cat no : 67985-1-Ig

Synonyms

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

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Validation Data Gallery

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  WB analysis using 67985-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A549 using 67985-1-Ig

  WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells.

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  WB analysis of HeLa using 67985-1-Ig

  WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells.

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  IF Staining of HeLa using 67985-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67985-1-Ig targets MON1B in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MON1B fusion protein Ag9920
• Full Name: MON1 homolog B (yeast)
• Calculated Molecular Weight: 547 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC024277
• Gene Symbol: MON1B
• Gene ID (NCBI): 22879
• RRID: AB_2918734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).