MN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24697-1-AP

Synonyms

MGCR, MGCR1, MGCR1 PEN, MN1

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Validation Data Gallery

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  WB analysis using 24697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24697-1-AP (MN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 24697-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24697-1-AP (MN1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, U2OS cells
• Positive IHC detected in: human breast cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

24697-1-AP targets MN1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MN1 fusion protein Ag20344
• Full Name: meningioma (disrupted in balanced translocation) 1
• Calculated Molecular Weight: 1320 aa, 136 kDa
• Observed Molecular Weight: 136 kDa
• GenBank Accession Number: BC156879
• Gene Symbol: MN1
• Gene ID (NCBI): 4330
• RRID: AB_2879678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC,IF	Nat Commun; Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.; Authors - Norman L Lehman
human	WB,IHC	EBioMedicine; METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.; Authors - Hong-Bo Li ; KD Validated