MMP8 Recombinant antibody

MMP8 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230030G11

Cat no : 82885-1-RR

Synonyms

CLG1, HNC, Matrix metalloproteinase 8, MMP 8, MMP8, Neutrophil collagenase, PMNL CL, PMNL collagenase

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Validation Data Gallery

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  WB analysis using 82885-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82885-1-RR (MMP8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HSC-T6 cells, K-562 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82885-1-RR targets MMP8 in WB, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: matrix metallopeptidase 8 (neutrophil collagenase)
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC074988
• Gene Symbol: MMP8
• Gene ID (NCBI): 4317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa,respectively(PMID: 28772283, PMID:15042023).