MMP19 Polyclonal antibody

MMP19 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14244-1-AP

Synonyms

matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 14244-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14244-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14244-1-AP

  IP result of anti-MMP19 (IP:14244-1-AP, 4ug; Detection:14244-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 14244-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using MMP19 antibody (14244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

14244-1-AP targets MMP19 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP19 fusion protein Ag5501
• Full Name: matrix metallopeptidase 19
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC050368
• Gene Symbol: MMP19
• Gene ID (NCBI): 4327
• RRID: AB_10637857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

Publications

Species	Application	Title
mouse	WB	Cancer Gene Ther; A critical ETV4/Twist1/Vimentin axis in Ha-RAS-induced aggressive breast cancer.; Authors - Wuling Liu
human	WB,IHC	J Cell Biochem; MicroRNA-193b-3p Regulates Matrix Metalloproteinase 19 Expression in Interleukin-1β-Induced Human Chondrocytes.; Authors - Zong-Kun Chang
human	WB	Front Pharmacol; METTL3/m6A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma; Authors - Fangfang Ge
human	WB	J Biochem Mol Toxicol; Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling; Authors - Wei Xu
rat	WB	J Immunol Res; Dissecting the Molecular Mechanism of Wang-Bi Capsule in the Treatment of Experimental Rheumatoid Arthritis Based on Synovial Tissue Proteomic Analysis.; Authors - Haiyang Shu