MINK1 Polyclonal antibody

MINK1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13137-1-AP

Synonyms

B55, GCK family kinase MiNK, hMINK, hMINKbeta, MAP4K6, MEK kinase kinase 6, MEKKK 6, MINK, MINK1, Misshapen like kinase 1, Misshapen/NIK related kinase, YSK2, ZC3

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Validation Data Gallery

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  WB analysis using 13137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13137-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13137-1-AP (MINK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13137-1-AP

  IP result of anti-MINK1 (IP:13137-1-AP, 4ug; Detection:13137-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lymphoma using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 13137-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13137-1-AP (MINK1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lymphoma tissue, human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13137-1-AP targets MINK1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MINK1 fusion protein Ag3795
• Full Name: misshapen-like kinase 1 (zebrafish)
• Calculated Molecular Weight: 1332 aa, 150 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC034673
• Gene Symbol: MINK1
• Gene ID (NCBI): 50488
• RRID: AB_10644151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP	Hum Mol Genet; Lung adenocarcinoma cell-derived exosomes promote M2 macrophage polarization through transmission of miR-3153 to activate the JNK signaling pathway; Authors - Lu Xu
rat	WB	Cardiovasc Ther; Effects of Vitamin D Deficiency on the Function of the Cardiac Autonomic Nervous System in Rats.; Authors - Xuemei Luo
mouse	IHC	Front Genet; MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma; Authors - Zhifeng Chen