MID1IP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MID1IP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 15764-1-AP

Synonyms

G12 like, Mid1 interacting protein 1, MID1IP1, MIG12, Protein STRAIT11499, STRAIT11499, THRSPL

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Validation Data Gallery

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  WB analysis of HEK-293 using 15764-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15764-1-AP (MID1IP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15764-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15764-1-AP (MID1IP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15764-1-AP targets MID1IP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MID1IP1 fusion protein Ag8400
• Full Name: MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))
• Calculated Molecular Weight: 183 aa, 20 kDa
• Observed Molecular Weight: 23 kDa, 46 kDa
• GenBank Accession Number: BC008908
• Gene Symbol: MID1IP1
• Gene ID (NCBI): 58526
• RRID: AB_2878181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MID1IP1, also named as Mid1-interacting protein 1 or Gastrulation-specific G12-like protein, is a 183 amino acid protein, which belongs to the SPOT14 family. MID1IP1 plays a role in the regulation of lipogenesis in liver. MID1IP1 may localizes in the nucleus and cytoplasm. MID1IP1 may form Homodimer in the absence of THRSP.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Acetylcorynoline Induces Apoptosis and G2/M Phase Arrest through the c-Myc Signaling Pathway in Colon Cancer Cells; Authors - Ye-Rin Park
human	WB	Biomolecules; Inhibition of CNOT2 Induces Apoptosis via MID1IP1 in Colorectal Cancer Cells by Activating p53.; Authors - Ji Hoon Jung ; KD Validated
human	WB,IF,IHC	Cells; Colocalization of MID1IP1 and c-Myc is Critically Involved in Liver Cancer Growth via Regulation of Ribosomal Protein L5 and L11 and CNOT2.; Authors - Ji Hoon Jung ; KD Validated
human	WB	Int J Mol Sci; The Antitumor Effect of Timosaponin A3 through c-Myc Inhibition in Colorectal Cancer Cells and Combined Treatment Effect with 5-FU or Doxorubicin; Authors - Hyun Min Ko
human	WB	Front Pharmacol; Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2; Authors - Hyun Min Ko
human	WB	Molecules; Euonymus sachalinensis Induces Apoptosis by Inhibiting the Expression of c-Myc in Colon Cancer Cells; Authors - So-Mi Park

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

kay (Verified Customer) (10-01-2022)

Very effective

• Applications: Western Blot