MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25109-1-AP

Synonyms

GlcNAc T IVa, GNT IV, GNT IVA, MGAT4A

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Validation Data Gallery

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  WB analysis of HeLa using 25109-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25109-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 25109-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25109-1-AP (MGAT4A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25109-1-AP is MGAT4A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT4A fusion protein Ag18956
• Full Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
• Calculated Molecular Weight: 535 aa, 62 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC127107
• Gene Symbol: MGAT4A
• Gene ID (NCBI): 11320
• RRID: AB_2879901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Biochim Biophys Acta Mol Cell Res; N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility; Authors - Depeng Yang