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  • KD/KO Validated

MFAP5 Polyclonal antibody

MFAP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15727-1-AP

Synonyms

MAGP 2, MAGP2, MFAP 5, MFAP5, MP25



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15727-1-AP targets MFAP5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFAP5 fusion protein Ag8467
Full Name microfibrillar associated protein 5
Calculated Molecular Weight 173 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC005901
Gene Symbol MFAP5
Gene ID (NCBI) 8076
RRIDAB_2266295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MFAP5 antibody 15727-1-APDownload protocol
IHC protocol for MFAP5 antibody 15727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Hepatology

YAP promotes proliferation, chemoresistance and angiogenesis in human cholangiocarcinoma via TEAD transcription factors.

Authors - Patricia Marti
  • KD Validated
humanIHC

Clin Transl Med

Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.

Authors - Qian Hao
humanIHC

Front Oncol

DPP6 and MFAP5 are associated with immune infiltration as diagnostic biomarkers in distinguishing uterine leiomyosarcoma from leiomyoma

Authors - Yumin Ke
humanIHC

Hum Cell

Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer.

Authors - Zhao Zhang
humanWB

Clin Transl Oncol

Stromal fibroblast-derived MFAP5 promotes the invasion and migration of breast cancer cells via Notch1/slug signaling.

Authors - Z Chen
  • KD Validated
humanIHC

Int J Mol Sci

Microfibril Associated Protein 5 (MFAP5) Is Related to Survival of Ovarian Cancer Patients but Not Useful as a Prognostic Biomarker

Authors - Katarzyna Aleksandra Kujawa