METTL6 Monoclonal antibody, PBS Only

METTL6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7D4

Cat no : 68400-1-PBS

Synonyms

methyltransferase like 6, METTL6

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Validation Data Gallery

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  WB analysis using 68400-1-Ig (same clone as 68400-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68400-1-Ig (METTL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68400-1-Ig (same clone as 68400-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68400-1-Ig (METTL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68400-1-PBS in a different storage buffer formulation.

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Product Information

68400-1-PBS targets METTL6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: METTL6 fusion protein Ag9714
• Full Name: methyltransferase like 6
• Calculated Molecular Weight: 255 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC022400
• Gene Symbol: METTL6
• Gene ID (NCBI): 131965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).