MEST Polyclonal antibody

• Featured Product
• KD/KO Validated

MEST Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11118-1-AP

Synonyms

MEST, PEG1

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Validation Data Gallery

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  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11118-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11118-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11118-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11118-1-AP (MEST antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11118-1-AP

  IP result of anti-MEST (IP:11118-1-AP, 3ug; Detection:11118-1-AP 1:500) with PC-3 cells lysate 1720ug.

• 

  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11118-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11118-1-AP (MEST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

11118-1-AP targets MEST in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEST fusion protein Ag1617
• Full Name: mesoderm specific transcript homolog (mouse)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC011908
• Gene Symbol: MEST
• Gene ID (NCBI): 4232
• RRID: AB_2143740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

Publications

Species	Application	Title
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse	IHC	Nucleic Acids Res; Tissue-specific alternative polyadenylation at the imprinted gene Mest regulates allelic usage at Copg2.; Authors - Julia L MacIsaac
human	IHC	Gynecol Oncol; Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.; Authors - Jing Zhang
human	WB	Cancer Lett; Novel target genes responsive to the anti-growth activity of triptolide in endometrial and ovarian cancer cells.; Authors - Li Haili H
human	WB,IHC	Placenta; DNA methylation-associated repression of MEST/PEG1 expression contributes to the invasion of extravillous trophoblast cells.; Authors - Wei Peng ; KD Validated
human	WB	Placenta; Maternal obesity and gestational weight gain are modestly associated with umbilical cord DNA methylation.; Authors - Keshari M Thakali