MED4 Monoclonal antibody

MED4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6F4

Cat no : 67839-1-Ig

Synonyms

ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP

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Validation Data Gallery

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  WB analysis using 67839-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67839-1-Ig (MED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67839-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67839-1-Ig (MED4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67839-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED4 antibody (67839-1-Ig, Clone: 2E6F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue).

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Tested Applications

• Positive WB detected in: LNCaP cells, Hela, HEK-293, Jurkat, K-562, HSC-T6, PC-12, NIH/3T3, 4T1
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67839-1-Ig targets MED4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MED4 fusion protein Ag8562
• Full Name: mediator complex subunit 4
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: ~36 kDa
• GenBank Accession Number: BC005189
• Gene Symbol: MED4
• Gene ID (NCBI): 29079
• RRID: AB_2918601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".