CoraLite® Plus 488-conjugated MED15 Polyclonal antibody

• Featured Product
• KD/KO Validated

MED15 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11566

Synonyms

ARC105, CAG7A, CTG repeat protein 7a, CTG7A, DKFZp686A2214, DKFZp762B1216, FLJ42282, FLJ42935, MED15, mediator complex subunit 15, PCQAP, TIG 1, TIG1, TNRC7, TPA inducible gene 1 protein

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Validation Data Gallery

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  IF Staining of SH-SY5Y using CL488-11566

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 MED15 antibody (CL488-11566) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11566 targets MED15 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED15 fusion protein Ag2143
• Full Name: mediator complex subunit 15
• Calculated Molecular Weight: 788 aa, 87 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC017110
• Gene Symbol: MED15
• Gene ID (NCBI): 51586
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).